Molecular authentication of Maytenus sp by PCR-RFLP
نویسندگان
چکیده
منابع مشابه
Molecular authentication of Panax ginseng species by RAPD analysis and PCR-RFLP.
In order to develop convenient and reproducible methods for the identification of ginseng drugs at a DNA level, randomly amplified polymorphic DNA (RAPD) and PCR-restriction fragment length polymorphism (PCR-RFLP) analyses were applied within Panax species. To authenticate Panax ginseng among ginseng populations, RAPD analysis was carried out using a 20 mer-random primer. The similarity coeffic...
متن کاملDNA Barcode-Based PCR-RFLP and Diagnostic PCR for Authentication of Jinqian Baihua She (Bungarus Parvus)
We established polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and diagnostic PCR based on cytochrome C oxidase subunit I (COI) barcodes of Bungarus multicinctus, genuine Jinqian Baihua She (JBS), and adulterant snake species. The PCR-RFLP system utilizes the specific restriction sites of SpeI and BstEII in the COI sequence of B. multicinctus to allow its cleavage ...
متن کاملIdentification of Cryptosporidium Sp. Oocysts in Water by Modified Immunomagnetic Separation and Rflp-pcr
Current methodologies for detection of Cryptosporidium sp. in water, despite some efforts for standardization (e.g. EPA Methods 1622/1623), has several drawback that limit the applicability or interpretation of the results of Cryptosporidium monitoring. Presence of some chemical dissolved in water, ageing of oocysts, reduced affinity of antibodies used in immunomagnetic separation (IMS) and imm...
متن کاملDifferentiation of Adulterated Meat Products through Molecular Technique: PCR-RFLP
Meat adulterations of different species are undetectable and it is common practice globally. In the field of food analysis, species determination is mostly sufficient, but simultaneous detection of several species in a single food product is desirable. The aim of the study was to distinguish between meats of two different species through PCR-RFLP analysis. The meat of two species were used incl...
متن کاملMolecular typing of avian Pasteurella multocida isolates from Iran by PCR-RFLP of OmpH gene
Molecular typing of twenty-five Pasteurella multocida isolates has been assessed by restriction fragment length polymorphism (RFLP) of a species-specific PCR assay. All of the P.multocida isolates were recovered from fowl cholera outbreaks in Northern provinces of Iran. Amplification was based on the gene ompH, encoding a major outer membrane protein (protein H). PCR with ompH primers amplified...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Semina: Ciências Agrárias
سال: 2013
ISSN: 1676-546X,1679-0359
DOI: 10.5433/1679-0359.2013v34n2p627